Biomarker Discovery:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
CRISPR:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Quantitative Proteomics:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Single Cell:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Clone Assay:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Control:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Western Blot:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Quantitative RT-PCR:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Virus:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Infection:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Plaque Assay:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Expressing:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Stable Transfection:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Plasmid Preparation:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Comparison:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Binding Assay:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Construct:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Transfection:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Affinity Purification:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Mass Spectrometry:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Protein-Protein interactions:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Staining:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Mutagenesis:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
SDS Page:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Modification:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Ubiquitin Proteomics:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Liquid Chromatography with Mass Spectroscopy:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Cryo-EM Sample Prep:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Activity Assay:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Purification:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Incubation:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
In Vitro:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Recombinant:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Labeling:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
RNA Binding Assay:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Sequencing:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Residue:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Knockdown:Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation.
Article Snippet: Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).Coimmunoprecipitations, SDS- PAGE, and IB analyses were carried out using published protocols (49, 53, 54) (SI Appendix, Methods).. Anti- TRIM32 antibody (10326- 1- AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14- ExoN (SARS- CoV- 2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS- CoV- 2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
Article Title: Inhibition of coronaviral exoribonuclease activity by TRIM-mediated SUMOylation
Article Snippet: Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).Coimmunoprecipitations, SDS-PAGE, and IB analyses were carried out using published protocols ( , , ) ( SI Appendix , Methods ).. Anti-TRIM32 antibody (10326-1-AP, Proteintech or GTX113937, GeneTex) was used at a dilution of 1:1,000.. The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.The Nsp14-ExoN (SARS-CoV-2) antibody was purchased from CST (#99098) and was validated in the Gack lab for its specific reactivity against Nsp14, but not other SARS-CoV-2 Nsps (i.e., Nsp2, Nsp10, Nsp15, and Nsp16), in cells transfected with plasmids encoding those proteins.
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